FIDA Advanced Course at Harwell (UK)

FIDA Advanced Courses are designed to teach key capabilities that improve both Fida 1 user experience and data quality. Whether you have been using FIDA for many years or you performed your first few assays, this course can greatly benefit you, as:

• You will gain the knowledge that will allow you to extract and interpret all the of information that is present in Fida 1 data
• You will learn about new features of the Fidabio software
• You will network with the global FIDA community
•  You will have a chance to address your specific issues with our scientists

Topics covered

• Assay optimization: labelling strategies, pressure control, etc
• Ensuring optimal data – from any kind of sample (hands-on session)
• Know the stability of your protein sample (hands-on session)
• User cases. The course gives you the chance to discuss your specific case.The program will be sent to the participants 1 week before the course.

Agenda:

Tuesday, June 20th

9:30 Arrival

10:00 Welcome and FIDA introduction (HJ)

10:10 Fida principles and best practice

11:15 Coffee Break

11:30 Tackling sticky sample (hands-on) Different capillary types, dynamic and permanent coating, analyzing sticky systems, such as lysozymes, protein-A, histones etc.

12:30 Viscosity effect & compensation for corrected Rh (EGPVS)

13:00 Lunch

14:00 Getting around the corners of the Fidabio Software Suite (EGPVS) Introduction to new features - PDI, PDB, LLPS, Spike counter, Denaturation standalone (useful for later hands-on), Sequence Excel-like function, Addition of saved sequence blocks.

15:00 Why first principle matters (HJ) Including an open discussion on how first principle makes a difference.

15:30 Coffee Break

16:00 Know the stability of your protein sample (hands-on) (EGPVS/HJ) Gn-HCl denaturation assay with HSA.

17:00 Case studies - Analysis of dataset from a user.

17:30 Wrap up

19:00 Dinner at Milton Hill Hotel

Wednesday, June 21st

9:30 Arival

10:00 Stability Data analysis on UV (Hands-on) Analyze the data from the day before - Protein stability Software.

11:15 Coffee Break

11:30 Method optimization (hands-on) (EGPVS/HJ) Pressure, mixing modes and capillary radii

12:30 Advanced applications (HJ) Examples of advanced Fida 1 applications

13:00 Lunch

14.00 System optimization

- Labelling strategies (EGPVS) Selection of coupling buffers and chemistries (NHS, maleimide, Tris-NTA) as well as optimal choice binding related intensity change (BRIC)

- Getting the most of your capillaries (HJ) Strategies to get the most out of your capillaries

- Pressure control (HJ) Ensuring the right pressure

16:00 Coffee & discussion on pending topics Fidabio tech support and contact information.

16:30 End of the course – apply your new learnings!

Course leaders:

Henrik Jensen, Phd. - Chief Scientific Officer and inventor of FIDA Technology

Emil G. P. Stender - Senior Field Application Scientist

Costs:

Participation in this Advanced Course is free.

Accommodation and travel costs are not covered. However, we can book accommodation for you, and you cover the cost after arrival. In such a case, Milton Hill House Hotel, with a B&B rate of £115pppn.